We extracted the active components of different segments (top,middle & basis) of six fresh velvet anflers-polypeptides with acetic acid solution(pH 3.5)and alcohol and analyzed by SDS-PAGE and Western blotting with anti-human IGF-1、EGF、NGF as the first antibodies.The total polypeptides and IGF-1 contents in different segments of velvet antler were detected with Coomassie brilliaat blue G-250 and RIA techniques.EGF and NGF OD values were compared with ELISA.Results:The results of SDS -PAGE indicated that dee...