The deacetyled Konjac glucomannan(d-KGM) sol-gel was prepared and the structural transformation from KGM to d-KGM was confirmed by FT-IR.The effects of experimental conditions related to the preparation of d-KGM sol-gel upon the properties of d-KGM membrane and the immobilization of enzyme were investigated.Glucose biosensor had been fabricated by immobilizing enzyme with d-KGM.The linear range of biosensor was 0.1 to 8 mmol/L with a sensitivity of 240 nA/mmol/L. The apparent Michealis-Menten constant is 19...