In this essay we took Streptomyces albulus B-215 as test bacterium,treated it with UV to select and breed High-producing Strain.Through UV mutation,we got the mutant BU-215 whose productivity of ε-polylysine was 0.75 g/L,which was 20.97% higher than the comparison.And its H/C of inhibitive circle was 1.56,which is 25.81% higher than the comparison.