[Objective] The research aimed to discuss the genetic diversity among 24 common wheat varieties at home and abroad. [Method] Improved phenol-chloroform method was used to extract genomic DNA from 24 common wheat varieties at home and abroad. Genomic-SSR and EST-SSR marker technologies were adopted to analyze the genetic diversity of 24 wheat varieties and two evaluation methods were analyzed and compared. [Result] When 37 pairs of Genomic-SSR premiers were used to amplify 38 sites in 24 wheat genotypes, 152...