AFLP(Amplified Fragment Length Polymorphism) silver-staining analysis system was established with 14 tobacco cultivars.The results of several reactions in the analysis system were as following: tobacco genomic DNA was extracted by CTAB method;the double enzymes restriction and the adapter-ligation were completed at the same time;E+A and M+C primer combinations were used in pre-amplification while E+3 and M+3 primer combinations in selective amplification;the PCR reaction products were analyzed on 6% denatur...
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