Objective The assimilating cholesterol abilities of Lactobacillus acidophilus were preliminarily studied in MRS liquid medium. Method Imitating human different cholesterol concentration. Result The abilities of assimilating low and normal cholesterol of Lactobacillus acidophilus were not apparent but the abilities of assimilating high cholesterol were obvious. Conclusion Lactobacillus acidophilus had the ability to assimilate cholesterol.