Spike detection in the research of hippocampal neural network electrophysiology with extracellular recording in vitro from Multi-microelectrode arrays (MEA) is an elementary pre-processing. Recent work detects spike through diversified algorithms such as threshold detection and peak-detection that is brought up. A revised version of the peak-detection algorithm is supposed. This advanced approach not only works out an unwanted detection problem of the previous one caused by the position of sliding window bo...