目的:探讨Survivin反义核酸对乳腺癌细胞增殖的影响.方法:本实验分6组,分别以脂质体介导反义寡核苷酸、无义寡核苷酸及RPMI 1640培养液作为空白的对照组,转染人乳腺癌MCF-7细胞系,采用Real time RT-PCR,Western blot方法检测各组MCF-7细胞Survivin蛋白及mRNA的表达情况,测定转染后细胞贴壁率变化,研究Survivin反义寡核苷酸对MCF-7细胞的生长抑制作用.结果:脂质体介导Sur-vivin反义寡核苷酸转染人乳腺癌MCF-7细胞后,细胞内Survivin蛋白及mRNA表达显著降低,通过Realtime RT-PCR检测MCF-7细胞经作用后其Survivin mRNA起始拷贝数明显下降;同时细胞贴壁率降低达32.96%,细胞生长抑制率最高达73.62%,ASOND/LiP组与NODN/LiP组和LiP组差异有显著性(P<0.05).结论:脂质体介导转染的反义寡核苷酸可在Survivin蛋白及mRNA水平有效地降低乳腺癌MCF-7细胞内Survivin的表达,并能够有效抑制乳腺癌细胞的增殖活性,提示Survivin靶向治疗可能成为乳腺癌综合治疗的一种重要方法...
【英文摘要】
Objective: To explore the influence of cell proliferation on human breast cancer after survivin was blocked with the antisense technique.Methods: The experiment was done in 6 groups.Antisense oligonucleotide(ASODN),nonsense oligonucleotide(NODN) were transfected in human breast cancer MCF-7 cell line and RPMI 1640 culture medium(normal control).Real time RT-PCR and western blot were applied in this study to detect and measure survivin protein and mRNA expression in MCF-7 cell.The cell adherent rate after tr...
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