γ-Aminobutyric acid(GABA) and L-aspartic acid(LAsp) were produced by means of biotransformation of the mixture of L-glutamic acid(L-Glu) and L-Asp in the presence of L-glutamate decarboxylase in Escherichia coli AS 1.505 which attacks solely on L-Glu.Under the conditions temperature 35 ℃,pH=5.0,ρ(baterium)=6 g/L,ρ(Tween80)=0.15 g/L,strain age 16 h,and ρ(substrate)=60 g/L,the specific activity was up to 15 036 U.1 g wet bacteria can convert 30 g mixture of L-Glu and L-Asp by repeatedly use for 3 times with 1...
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