Monolithic column is a new medium for chromatographic separation and analysis. Compared with the conventional particulate materials, the new material has many advantages such as low backpressure at chromatographic runs, high resolution and large capacity for solutes. In this paper, the polymer monolithic column and its applications for the chromatographic separation of bio-molecules such as peptides and proteins are introduced. In addition, the problem and the prospects of its applications are also proposed...