用不同浓度配比的生长素和细胞分裂素诱导梨枣叶片和茎段愈伤组织的产生,并研究了不定芽诱导的最佳配方,建立了梨枣叶片和茎段的再生体系。结果表明,梨枣叶片愈伤组织诱导的最佳培养基为M S+2,4-D 1.5m g.L-1+6-BA 0.5 m g.L-1;茎段为M S+2,4-D 1.0 m g.L-1+6-BA 0.5 m g.L-1。叶片不定芽诱导的最佳培养基为M S+IBA 0.1 m g.L-1+6-BA 1.5 m g.L-1。A gNO3能阻止叶片外植体褐化并有效地促进叶片愈伤组织分化。茎段能在同一培养基上产生愈伤组织并直接分化出不定芽。
【英文摘要】
Different concentration combinations of the growth regulators,2,4-D,6-BA and IBA were adopted to induce the calluses from the leaves and stems of Chinese date,then explore the optimal concentration formula of the growth regulators for adventitious bud induction and finally construct the system for Chinese date to regenerate from its leaves and stems.The results showed that the optimal medium for inducing calluses from the leaves of Chinese date was MS+1.5 mg·L~(-1) 2,4-D + 0.5 mg·L~(-1) 6-BA;the optimal med...
【基金】
山西省科技攻关项目(041035)
【更新日期】
2005-11-25
【分类号】
S665.1;
【正文快照】
Ch inese date(Z izyphus jujuba M ill.)is an e-conom ically im portant fru it tree cu ltivated innorthern Ch ina for its ed ib le fru it,d istributingfrom 23°N to 42°N and from 76°E to 124°E.Ch i-nese date cu ltivar L izao orig inated from Y uncheng,Sh
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