Using Agrobacterium rhizogene ATCC15834,the study transformed Reynoutria japonica to probe the factors affecting the induction of its hair-like roots.The results revealed that the mature leaf blades were the explants with high transformation frequency,which could forms hair-like roots about ten days after the inoculation with their induction rate averaging 55.66% and their root density standing at(4.30).It was proved by PCR test that the genes rolB and tms in Ri plasmid of Agrobacterium rhizogene were compl...
【基金】
河北省自然科学基金资助(C2005000741)
【更新日期】
2005-11-25
【分类号】
S567.239;
【正文快照】
虎杖(R eynoutria japon ica Houtt.)为多年生蓼科草本植物,主要分布于秦岭以南地区,根或根茎入药,其主要有效成分是芪类化合物和蒽醌类化合物,其中芪类化合物主要包括白藜芦醇和虎杖苷.药理学研究表明,白藜芦醇及其苷类是一类具有多种药理作用的活性化合物[1],对癌变过程中细?
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