Bupleurum longiradiatumTurcz. were used for inducationg of calli in vitro. Genetic differences between wild plantlets and calli had been analysed on DNA level with the application of random amplified polymorphic DNA(RAPD) technology to the molecular genetic marker. The fingerprints amplification by polymerase chain reaction(PCR) with 7 polymorphic 10-based random primers selected from 13 ones was analysised. The results indicated that there were genetic variation between wild plantlet and calli.