Microwave-assisted extraction of hippophae rhamnoides L. leaves polysaccharide was investigated with orthogonal array design; the factors of liquid-solid radio, microwave power and extracting time are selected. The results demonstrated that the optimum extraction conditions are liquid-solid ratio of 1∶40(g∶mL), microwave power of 540 W,extracting time of 50 s and the extracting rate of hippophae rhamnoides L. polysaccharide come to 5.25%.