Aim To discuss the method of measuring vitamin C in the pears and apples on spectrophotometer. Methods Add oxalic acid to the await measuring sample to deal with into the even thick liquid, then use spectrophotometry for measuring it in 490 nm after filtering. Results Through the spectrophotometer scanning, the peak value of the osazone is admitted as 488~492 nm, temperature is the important influence factor in the course. Conclusion This method can be used in the survey of vitamin C in the pears and ap...
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