Genetic transformation system of Amorphophallus wa s established via gene-gun .Results showed that the optimum helium gas pressure/b ombardment distance was 7.58 Gpa/9 cm. MS medium with addition of 0.4 mol/L man nit was used to do hypertonic treatment 4 hours before bombardment and 16 hours after bombardment . This could decrease adversity damage and increase the probab ility of transformation success. To obtain transgenic plants during screening,th e selected pressure was 1 mg/L Basta at the early stag...
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