<正> The multiplex polymerase chain reaction (PCR) technique was applied to detect the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) specific target cDNA fragments in the present study. The target cDNA fragments of SARS-CoV were synthesized artificially according to the genome sequence of SARS-CoV in GenBank submitted by The Chinese University of Hong Kong, and were used as simulated positive samples. Five primers recommended by World Health Organization (WHO) were used to amplify the f...
【英文摘要】
The multiplex polymerase chain reaction (PCR) technique was applied to detect the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) specific target cDNA fragments in the present study. The target cDNA fragments of SARS-CoV were synthesized artificially according to the genome sequence of SARS-CoV in GenBank submitted by The Chinese University of Hong Kong, and were used as simulated positive samples. Five primers recommended by World Health Organization (WHO) were used to amplify the fragm...
【基金】
The Science Founda-
tion of Fujian Entry-Exit Inspection and Quarantine Bureau (FK2003-35).
【更新日期】
2005-08-19
【分类号】
R346
【正文快照】
IntroduetionIn Novexlzber 2002,the severe aeute resPiratory syn-elrome(SARS)emerged in Guangdong Province,China,and spreaded raPidly to other areas in Chinaand more七hajl 20 eountries elsewhere in the丫v〔)rld.]口housands of People were infeeted and hundr
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