Hepatitis B virus(HBV) is one of the most important human pathogens. HBV polymerase has a central role in the replication of the virus. The full-length polymerase gene from HBV-56 was cloned, and expressed with the recombinant adenovirus system. After the infection of HEK293 cells with recombinant adenovirus, the expression of HBV polymerase was observed. A semi-quantitative PCR was developed and used to demonstrate the reverse transcriptase activity of the recombinant protein. This work may contribute to t...