Huai Rehmannia glutinosa Libosch. genomic DNA,extracted from its fresh young leaves by CTAB method,was used as ISSR-PCR amplification template.The effects of main elements,i.e.temperature,Taq polymerase dosage,dNTP concentration,Mg~(2+) concentration,primer concentration and template DNA concentration on ISSR-PCR amplication were tested by single factor experiment,respectively,to determine its optimal levels and establish the following optimal reaction system for ISSR analysis in Huai Rehmannia glutinosa Li...
【更新日期】
2005-08-19
【分类号】
Q943
【正文快照】
ISSR inter- simple sequence repeat is a newkind of DNA markers putforward by Zietkiewiczetal[1] 1 994 .It is a technique in which primers an-nealing to microsatellites MS during PCR lead tamplification of the piece of DNA between twoprimers that are wit