Organism BRF-1(Bacillus spp. ) was isolated from soybean rhizosphere. Dual-culture tests showed that it had strong antifungal activity to 4 pathogens of soybean root-rot disease. Further pot experiment indicated it would be a hopeful bio-control strain because it was not only to inhibit the soybean root-rot disease caused by Fusarium oxysporum but also promoted soybean seedling.