Karyotype in three populations(P_1,P_2,P_3) of lilium nepalense D.Don was analyzed in this paper. All the populations exist and are scattered in Yunnan. The karyotypical variation patterns among the populations,individuals,cells,homologous chromosomes were respectively studied in chromosomal level by using routine statistic and nested analysis.The nested analysis of variance shows that about 4.5% of the chromosomal variability is attributed to the differentiation among populations and the remaining 95.5% wi...