Objective:To explore the role of the real time fluorescence quantitative polymerase chain reaction(FQ PCR) in detecting heptitis B virus(HBV) DNA.Methods:The sera from 196 patients were measured by FQ PCR.Results:In the 112 HBeAg(+)/HBeAb(-) samples,the FQ PCR results were all positive,with 1.08×10 7/ml of HBV DNA on the average.In the 68 HBeAg(-)/HBeAb(+) samples,the positive rate was 66.2 % with a duplication amount of 6.12×10\+5/ml on the average.In the 16 HBeAg(-)/HBeAb(-) samples,the duplica...