In order to improve propagation rate of Dendrobium Huoshanense, the experiment in micro-propagation technology of tube seedling in Dendrobium Huoshanense was conducted. The results showed: different plant growth regulators and concentration combination, different sugar amounts and pH values had obverious effect on tube seedling growing and propagation. Among those, the best combination was MS+KT*!3*! mg/L +2.4-D*!0.2*!mg/L + sugar 3% with pH=5.4.The best transplanting medium was cortex.The better was co...