Objective A good method is presented for neonatal rat Schwann cell in vitro. The experiments conducted in rats were aimed at clinical use. Methods Neonatal rat sciatic nerve explants were repeatedly moved and stimulators forskolin and BTI were simultaneously added into the medium in culture. Immunofluorescent staining was used to observe the glia-specific marker S100, as a positive marker for Schwann cell. The method with an in situ cell proliferation kit was processed to test BrdU incorporation. Result...