Objective: To study the stimulation effects of hCG on the Leydig cells cultured in vitro. Methods: Leydig cells were randomly selected from isolated human testis to primarily culture and subcultur. The same sample was divided into 4 groups. The Ley-dig cells were stimulated with hCG on different periods and the culture fluids collected to examinate the testoterone by SEROZYME system. Results: The primarily cultured and subcultured Leydig cells were sensitive to hCG in first stimulation but the secretion of ...