An isolation method of adult rabbit cardiomyocytes (two steps collagenase perfusion method) and cardiomyocyte culture after isolation were introduced in detail. According to experience from practice, it is emphasized that main causes of fault is too deep insertion of the perfusion tube end and collagenase dosage is improper. It is noted that the pH of perfusion and collagenase fluids should be kept between 7 35 and 7 45, and the temperature of perfusion fluid should be kept at 37℃, so that the damaged a...
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