This paper has summarized the plant DNA isolation methods which were used as routine protocols in Hainan Provincial Key Laboratory of Crop Molecular Breeding (HiCMBL) All of the protocols originated in many famous domestic and international laboratories and modified by HiCMBL It is well adapted for applications of the lab of molecular plant breeding, those protocols are quick, reliable and the DNA obtained can be met the demands of the researches in modern molecular biology and biotechnology