Take the pteridophyte in Qingyuan Mountain as sample, compate extractive methods and condition of total flavonoids from medicinal pteridophyte.It was researched that the extractor, extraction solvent, chromatography material had influence upon yield of total flavonids. The experimental results showed that with 70% ethanol or 60% acetone was used as solvent, Soxnet extractor, polyamide chromatography or silica gel chromatography is the best method of extraction flavone from peridophyle.