A high performance liquid chromatorgaphic method for the determination of thiophene in scorched benzene has established with the mobile phase of methanol-water, including the ratio of mobile phase, current velocity, column temperature and so on. Determinating Thiophene in scorched benzene:mobile phase methanol-water 60:40(0.0 min) -80:20(20.0 min),0.8 mL/min, wave-length 220 nm,column temperature 30C . Better separation was gotten from the mixture, and determining the precision and accuracy.
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