Rapid amplification of cDNA ends (RACE) is a polymerase chain reaction (PCR)-based technique which was developed to facilitate the cloning of full-length cDNA 5' and 3' ends after a partial cDNA sequence has been obrained by other methods. While RACE can yield complete sequences of cDNA ends in only a few days, the procedure frequently results in the exclusive amplification of truncated cDNA ends, undermining efforts to generate full-length clones. Many investigators have suggested modifications to the RACE...
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