The progress of crop breeding can be promoted via doubled haploid technique that can rapidly polymerize and purify several advanced genes The virescently marked semigamy line(vSg) in island cotton (Gossypium barbadense L) has potential to produce high frequency of haploids,and can be used as female parent to induce target haploids In the present study, the haploids of advanced hybridization of Bt transgenic upland cotton (Gossypium hirsutum L) were successfully induced by vSg It is difficult to ide...