By comparing cellulase activity of different strains, Trichoderma reesei Rut C-30 was found to be the best one. A combination of Avicel and wheat bran was used as carbon source, as well the KH 2PO 4-K 2HPO 4 buffer system was used for pH control, resulted in very high enzyme production in shake flask culture, Maximum CMCase 100-125U/ml, FPA 9-12U/ml were achieved within 6 days culture. When it was grown in 2.5L fermentor, where pH and DO levels were controlled, the enzyme activity were 133.4U/ml(CMCase)...
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