We utilized the protoplast fusion techinique,ultroviole light and EMS to induce original strain,and got the mutant GLR513 next we made research for the best culture conditions for lipid fermentation.The result is the best carbon source is glucose,the best nitrogen source is (NH4)2SO4,C:N is 70:1,initial pH is 5.5,and it is necessary to maintain this value during fermentation,the inoculation volume is 20%,optimum temperature is 30℃,fermentation time is 72h,during fermentation more glucose need.At the e...
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