The chromosomal position effect was tentatively investigated in Saccharomyces cerevisiae using the yeast invertase gene(SUC2). The SUC2 gene was integrated into different chromosomal loci via homologous recombination, FLP-FRT site-specific recombination and targeted integration. Its expression in the resultant transformed cell lines was then measured. The result shows that position effect exists in yeast but influences the expression of SUC2 gene quantitatively.