Genetic diversity of the 18 alfalfa local varieties in China and the 9 alfalfa varieties repre-senting the 9 basic alfalfa germplasm sources in North America were studied using seed storage proteinmarkers. Results showed that: Obvious polymorphisms within and among varieties can be revealed usingelectrophoresis of alfalfa single seed samples. Meanwhile, polymorphisms within varieties are concealedwhen using bulk samples. Monomer weights of alfalfa seed storage proteins are 21. 7 ~ 83. 8KD. 25 stableand dist...