Canadian Rambler alfalfa cultivar was introduced from Saskatchewan of Canada to Grass-land Research Institute of Chinese Academy of Agricultural Sciences in 1972. After improved and devel-oped for several years, it was registered by the Chinese Herbage Cultivar Registraion Board as a new cul-tivar in 1987. Through field trial and laboratory analysis, we found that genetic variation between two culti-vars by use of total 45 plants for each cultivar to evaluate the morphological, physiological and biochemical...