Studies on tissue culture of Stylosanthes guianensis REYAN Ⅱshow that cotyledon is apromising explant. Callus and shoots can be induced from cotyledon cultured on the medium containingbasic salt of MS, 0.8% agar, 3 .0% sucrose, 1 .0mg/L NAA and 4.0mg/L BA, pH6.0. Rooting canbe induced from one-month-old shoots cultrued on the mediurn containing half strength MS basic salt,0.8% agar, 1 .0% sucrose, 0.5mg/L NAA, and 0.005% active cbarcoal, pH6.0 and the rooting rateis 60%, 25ug/ml, 50ug/ml of Kanamycin inhibi...