Through systematical process for isolation and purification of laccase from fresh fruit body of Lenlinula edodes using the technique (NH 4) 2SO 4 fractionation and ionexchange chromatography.We obtained one component of laccase,it showed homogeneity in the gel electrophorosis.The molecular weight was 55 000 by SDS PAGE,The optimum pH, temparature were 2.6 ℃ and 70 ℃,respectively. The studies of enzyme kinetics showed K m =0 142 mmol