Objective: to set up a test methodology of determining MIC of dermatophytes by using a microdilution test, and provide a basis for the standardized tests in future. Methods: using 96 well microtiter trays as testing carriers, and ten twofold serial dilutions were made. The susceptibilities of 30 dermatophyte strains isolated from clinic to itraconazole and fluconazole, two new azole antifungal drugs, were determined. Results: the MIC range of itraconazole and fluconazole against 30 dermatophyte strains are...