The separation and quantitaitve analytical methods of tea Carotenoids were studied by HPLC.Results showed that four kinds of main tea carotenoids, namely β carotene, lutein, violaxanthin and neoxanthin, could be well separated in 10 min by extracting the sample in hexance∶acetone∶ethanol∶toluene(10∶7∶6∶7) and 40% methanolic potassium hydroxide for 12 h with Nova Pak C 18 stainless steel column(3.9×150 mm), using acetonitrile∶chloroform∶ tert butyl alcohol(83∶15∶2) as the mobile phase and detectin...