Colimitation to the biochemical composition and propagation of marine diatom Skeletonema costatum by interaction of environmental factors including Fe ( Ⅲ ) , N, Mn, light and temperature was studied by laboratory simulation. Three topics were discussed: 1)the relation of the biochemical composition and energetic limitation to the interaction of Fe3+ and light, Fe3+ and temperature at low available iron concentration; 2)the effect of the interaction of Fe ( Ⅲ ) and N/ P, Fe3+ and Mn2+ , Fe3+ and Cu2+ , Cu2+...