A chiral bonded stationary phase having Luhydroxy proline as chiral selector was prepared and the bonded phase was characterized by IR spectrometry, elemental analysis and nitrogen absorption at low temperatufe. The effects of the chromatographic conditions, such as pH and the ionic strength of the mobile phase and the column temperature, on the enan tiomeric resolution of the DL-α-amino acids were studied. The thermodynamics in chromatographic separation and the mechanism of enantiomeric resolution were di...