Chorismate mutase (CM ), prephenate dehydratase (PD ), and phenylalanine aminotransferase (PAT) are the three key enzymes in the fermentation of phenylalanine. In E. colt,the first two are encoded by a single gene pheA,while the third by tyrB gene. PheA and tyrB genes were cloned from an E. colt mutant strain resistant to mfluro-L-phenylalanine by polymerase chain reaction (PCR). These genes were expressed either separately or linked as an operon under lamda phage promotor PR in E. colt. Distinct bands w...
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