The intraverious and intrasplenic immunization method was used in the production of anti-Lseh -mania donouani monoclonal antibodies. The course of immunization in the BALB/C mice was divided into 5stages. From the first to third,the antigen was injected directly into the venous of the mice. At the fourth andfifth stage,without openning abdominal cavity,just opened the skin and directly injected the antigen into thespleen. The dose of injection of Leshmania donouani promastigotes was 0.5～1×107 per time,on...
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