The control of hydrogen sulfide using biological fixed-film filter is an economical and effective method. The experimental conditions were temperature of 20~30℃ and water spray quantity of 600l/m ̄3(packing)·d. 100% of H2S removal efficiency was obtained at the loading rate≤560gH2S/m3·d with Cin≤230mg/m3 (infuent H2S concentration). However, up to 98% of H2S removal efficiency was obtained at the loading rate≤2430gH2S/m3·d with Cin≤570mg/m3 and Cout≤10mg/m3.