A centrifugal enhancement and rapid plaque formation assay for respiratory syncytial virus(RSV)has been established, by inoculating the suspended vero cells and RSV into 24-well plate simutaneously,with methyl cellulose as the overlay medium.Centrifugal enhancement means that application of virus to suspended cells with the aid of a centrifugal field results in an increases number of plaques. The centrifugal enhancement effect was positively related with centrifugal force andtime.The plaque formation was al...