The effects of nutritional conditions on synthesis of lignin peroxidase and its isoenzymes by Phanerochate chrysosporium ME-446 were studied. Under the optimal culture conditions, the activity of 1 500U/L was obtained. Of the five isoenzymes separated with high performance liquid chromatergraphy,P2 content is the highest. Low carbon and high nitrogen medium is most suitable for synthesis of the enzyme. Lower contents of nitrogen or KH2PC)4 make decrease of all iosenzymes, while changing concentrations of Mg...