The lively bacterial preparation-Bacillus B01 has been used for chick feed additive. A total of 320 broilers was used in 3 conducted tests. The effect of gut bacterial flora was done. The results shew that average gain of treated groups increased 15. 56% compared with the control groups (P<0. 01), feed conversion rate decreased 15. 68% compared with the control groups (P<0. 05). The preparation also had objective effective, for preventive pullorosis, and had decreased the quantity of E. coli in the gut of ...