Highly efficient clonal propagation system was established via flower bud culture. MSmedium added with NAA 4 mg· l-1 and BA 5.5 mg·l-1 was suitable for callus induction.Adventitious shoots grew vigorously on MS medium supplemented with NAA 1 mg·l-1 and BA1 mg·l-1. Rooting of shoots was induced by transferred to MS medium withno hormones.This technique with 16348/250ds propagation rate kept the diploid ofWelsh onion.