The experiment was designed orthogonally involving 3 factors: different quantity of fertilizer applied, maturity degree and number of leaves harvested with field trial and sandy culture. During ripening of the plant, the total nitrogen, nicotine fructose, sucrose, total water soluble sugar, starch, semicellulose and dynamics of ni-trate reductase and amylase isoenzyme were measured for the assessment of relations between the substances metabolism and the quality formation, and selecting optimal cultural pra...